In laser applications such as optical tweezers and laser cutting, laser beams entering the rear aperture of an infinity objective can be tightly focused to a diffraction-limited spot, providing concentrated optical power and excellent resolution. As an advantage over finite conjugate objective lenses, a variety of auxiliary optical components, such as optical filters and polarizers can be inserted between the infinity objective lens and the tube lens without altering how the beam propagates and forms the image down the optical path. Learn to identify microscope objectives and their specialized properties by deciphering the engravings on the barrel. When a tube lens of a different focal length is used, the magnification will need to be adjusted accordingly. Microscope manufacturers offer a wide range of objective designs to meet the performance needs of specialized imaging methods, to compensate for cover glass thickness variations, and to increase the effective working distance of the objective. The labeled magnification is calculated, assuming the objective is being used with a tube lens of a particular focal length by design. Light rays leaving the objective’s rear aperture are collimated, so that for imaging applications, a secondary lens (usually called a tube lens) is needed in order to focus the collected light from the specimen onto the sensor. Infinity corrected objectives are used in a wide variety of imaging and laser focusing applications.
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